Mitochondrial Membrane Potential in Living Neurons Measured by Flim
نویسندگان
چکیده
منابع مشابه
Spontaneous changes in mitochondrial membrane potential in cultured neurons.
Using the mitochondrial membrane potential (DeltaPsi(m))-sensitive fluorescent dyes 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide (JC-1) and tetramethylrhodamine methyl ester (TMRM), we have observed spontaneous changes in the DeltaPsi(m) of cultured forebrain neurons. These fluctuations in DeltaPsi(m) appear to represent partial, transient depolarizations of indivi...
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Interactions between proteins can be demonstrated by fluorescence resonance energy transfer (FRET) [1]. The proteins under test must be fused with donor and acceptor molecules. At FRET energy is transferred non-radiatively from donor to acceptor. FRET can be detected in two ways: the change in emission-ratio between donor and acceptor can be measured, or the decrease in lifetime of the fluoresc...
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Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles of XTH2 cells exhibited three-exponential decay kinetics. Based on DASPMI photo...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.1574